8fiz

Electron Microscopy
3.8Å resolution

Cryo-EM structure of E. coli 70S Ribosome containing mRNA and tRNA (in the transcription-translation complex)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 56-mer (preferred)
PDBe Complex ID:
PDB-CPX-218045 (preferred)
Entry contents:
51 distinct polypeptide molecules
5 distinct RNA molecules
Macromolecules (56 distinct):
Small ribosomal subunit protein bS20 Chain: AB
Molecule details ›
Chain: AB
Length: 87 amino acids
Theoretical weight: 9.71 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7U7 (Residues: 1-87; Coverage: 100%)
Gene names: JW0022, b0023, rpsT
Sequence domains: Ribosomal protein S20
Small ribosomal subunit protein uS12 Chain: AC
Molecule details ›
Chain: AC
Length: 124 amino acids
Theoretical weight: 13.77 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7S3 (Residues: 1-124; Coverage: 100%)
Gene names: JW3304, b3342, rpsL, strA
Sequence domains: Ribosomal protein S12/S23
Small ribosomal subunit protein uS19 Chain: AD
Small ribosomal subunit protein uS13 Chain: AE
Small ribosomal subunit protein uS14 Chain: AF
Small ribosomal subunit protein uS3 Chain: AG
Small ribosomal subunit protein uS10 Chain: AH
Small ribosomal subunit protein uS4 Chain: AI
Small ribosomal subunit protein uS2 Chain: AJ
Small ribosomal subunit protein uS9 Chain: AK
Small ribosomal subunit protein uS7 Chain: AL
Small ribosomal subunit protein uS11 Chain: AM
Small ribosomal subunit protein bS6 Chain: AN
Small ribosomal subunit protein bS16 Chain: AO
Small ribosomal subunit protein uS5 Chain: AP
Small ribosomal subunit protein uS8 Chain: AQ
Small ribosomal subunit protein uS15 Chain: AR
Small ribosomal subunit protein uS17 Chain: AS
Small ribosomal subunit protein bS18 Chain: AT
Small ribosomal subunit protein bS21 Chain: AU
Large ribosomal subunit protein bL31 Chain: AV
Large ribosomal subunit protein uL2 Chain: BD
Large ribosomal subunit protein uL3 Chain: BE
Large ribosomal subunit protein uL4 Chain: BF
Large ribosomal subunit protein uL5 Chain: BG
Large ribosomal subunit protein uL18 Chain: BH
Large ribosomal subunit protein bL33 Chain: BI
Large ribosomal subunit protein uL6 Chain: BJ
Large ribosomal subunit protein bL9 Chain: BK
Large ribosomal subunit protein uL11 Chain: BL
Large ribosomal subunit protein bL32 Chain: BM
Large ribosomal subunit protein bL34 Chain: BN
Large ribosomal subunit protein bL35 Chain: BO
Large ribosomal subunit protein bL36A Chain: BP
Large ribosomal subunit protein uL30 Chain: BQ
Large ribosomal subunit protein uL13 Chain: BR
Large ribosomal subunit protein uL14 Chain: BS
Large ribosomal subunit protein uL15 Chain: BT
Large ribosomal subunit protein uL16 Chain: BU
Large ribosomal subunit protein bL17 Chain: BV
Large ribosomal subunit protein bL19 Chain: BW
Large ribosomal subunit protein bL20 Chain: BX
Large ribosomal subunit protein bL21 Chain: BY
Large ribosomal subunit protein uL22 Chain: BZ
Large ribosomal subunit protein uL23 Chain: DA
Large ribosomal subunit protein uL24 Chain: DB
Large ribosomal subunit protein bL25 Chain: DC
Large ribosomal subunit protein bL27 Chain: DD
Large ribosomal subunit protein bL28 Chain: DE
Large ribosomal subunit protein uL29 Chain: DF
Large ribosomal subunit protein uL10 Chain: DG
16S ribosomal RNA Chain: AA
Messenger RNA Chain: AW
23S ribosomal RNA Chain: BA
P-site tRNA Chain: BB
5s rRNA Chain: BC

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-29214