8olr

X-ray diffraction
2.8Å resolution

Structure of yeast 20S proteasome in complex with the natural product beta-lactone inhibitor Cystargolide A

Released:
Model geometry
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Function and Biology Details

Reaction catalysed:
Cleavage of peptide bonds with very broad specificity.
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 28-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149135 (preferred)
Entry contents:
14 distinct polypeptide molecules
Macromolecules (14 distinct):
Proteasome subunit alpha type-2 Chains: A, O
Molecule details ›
Chains: A, O
Length: 250 amino acids
Theoretical weight: 27.19 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P23639 (Residues: 1-250; Coverage: 100%)
Gene names: PRE8, PRS4, YML092C
Sequence domains:
Proteasome subunit alpha type-3 Chains: B, P
Molecule details ›
Chains: B, P
Length: 258 amino acids
Theoretical weight: 28.75 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P23638 (Residues: 1-258; Coverage: 100%)
Gene names: PRE9, PRS5, YGR135W
Sequence domains:
Proteasome subunit alpha type-4 Chains: C, Q
Proteasome subunit alpha type-5 Chains: D, R
Proteasome subunit alpha type-6 Chains: E, S
Probable proteasome subunit alpha type-7 Chains: F, T
Proteasome subunit alpha type-1 Chains: G, U
Proteasome subunit beta type-2 Chains: H, V
Proteasome subunit beta type-3 Chains: I, W
Proteasome subunit beta type-4 Chains: J, X
Proteasome subunit beta type-5 Chains: K, Y
Proteasome subunit beta type-6 Chains: L, Z
Proteasome subunit beta type-7 Chains: M, a
Proteasome subunit beta type-1 Chains: N, b

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 136.96Å b: 302.05Å c: 146.01Å
α: 90° β: 113.09° γ: 90°
R-values:
R R work R free
0.185 0.182 0.208