8p94

Electron Microscopy
3.3Å resolution

Cryo-EM structure of cortactin stabilized Arp2/3-complex nucleated actin branches

Released:

Function and Biology Details

Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 30-mer (preferred)
PDBe Complex ID:
PDB-CPX-237934 (preferred)
Entry contents:
12 distinct polypeptide molecules
Macromolecules (12 distinct):
Actin-related protein 3 Chain: A
Molecule details ›
Chain: A
Length: 418 amino acids
Theoretical weight: 47.43 KDa
Source organism: Homo sapiens
Expression system: unidentified baculovirus
UniProt:
  • Canonical: P61158 (Residues: 1-418; Coverage: 100%)
Gene names: ACTR3, ARP3
Sequence domains: Actin
Actin-related protein 2 Chain: B
Molecule details ›
Chain: B
Length: 394 amino acids
Theoretical weight: 44.82 KDa
Source organism: Homo sapiens
Expression system: unidentified baculovirus
UniProt:
  • Canonical: P61160 (Residues: 1-394; Coverage: 100%)
Gene names: ACTR2, ARP2
Sequence domains: Actin
Actin-related protein 2/3 complex subunit 1B Chain: C
Actin-related protein 2/3 complex subunit 2 Chain: D
Actin-related protein 2/3 complex subunit 3 Chain: E
Actin-related protein 2/3 complex subunit 4 Chain: F
Actin-related protein 2/3 complex subunit 5-like protein Chain: G
Actin, cytoplasmic 1 Chains: H, J, K, N, O, P, Q, R, S, W
Src substrate cortactin Chain: I
F-actin-capping protein subunit alpha-1 Chain: U
F-actin-capping protein subunit beta Chain: V
Phalloidin Chains: a, b, c, d, h, i, j, k, l, m

Ligands and Environments

2 bound ligands:
2 modified residues:

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-17558
Expression systems:
  • unidentified baculovirus
  • Escherichia coli