8uhd

Electron Microscopy
2.8Å resolution

Structure of paused transcription complex Pol II-DSIF-NELF - post-translocated

Released:

Function and Biology Details

Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 21-mer (preferred)
PDBe Complex ID:
PDB-CPX-279026 (preferred)
Entry contents:
18 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (21 distinct):
Negative elongation factor E Chain: X
Molecule details ›
Chain: X
Length: 380 amino acids
Theoretical weight: 43.32 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P18615 (Residues: 1-380; Coverage: 100%)
Gene names: NELFE, RD, RDBP
Sequence domains: RNA recognition motif
Transcription elongation factor SPT5 Chain: Z
Molecule details ›
Chain: Z
Length: 1087 amino acids
Theoretical weight: 121.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O00267 (Residues: 1-1087; Coverage: 100%)
Gene names: SPT5, SPT5H, SUPT5H
Sequence domains:
Negative elongation factor C/D Chain: W
Negative elongation factor B Chain: V
DNA-directed RNA polymerase subunit Chain: A
DNA-directed RNA polymerase subunit beta Chain: B
DNA-directed RNA polymerase RpoA/D/Rpb3-type domain-containing protein Chain: C
RNA polymerase Rpb4/RPC9 core domain-containing protein Chain: D
DNA-directed RNA polymerase II subunit E Chain: E
DNA-directed RNA polymerases I, II, and III subunit RPABC2 Chain: F
DNA-directed RNA polymerase subunit Chain: G
DNA-directed RNA polymerases I, II, and III subunit RPABC3 Chain: H
DNA-directed RNA polymerase II subunit RPB9 Chain: I
DNA-directed RNA polymerases I, II, and III subunit RPABC5 Chain: J
DNA-directed RNA polymerase RBP11-like dimerisation domain-containing protein Chain: K
RNA polymerase II, I and III subunit K Chain: L
Negative elongation factor A Chain: U
Transcription elongation factor SPT4 Chain: Y
Non-template DNA Chain: N
Template DNA Chain: T
RNA Chain: P

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.8Å
Relevant EMDB volumes: EMD-42270
Expression systems:
  • Trichoplusia ni
  • Escherichia coli