Function and Biology Details
Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Structure analysis Details
Assembly composition:
hetero 21-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-279026 (preferred)
Entry contents:
18 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (21 distinct):
Negative elongation factor E
Chain: X
Molecule details ›
Chain: X
Length: 380 amino acids
Theoretical weight: 43.32 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
Sequence domains: RNA recognition motif
Length: 380 amino acids
Theoretical weight: 43.32 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
- Canonical: P18615 (Residues: 1-380; Coverage: 100%)
Sequence domains: RNA recognition motif
Transcription elongation factor SPT5
Chain: Z
Molecule details ›
Chain: Z
Length: 1087 amino acids
Theoretical weight: 121.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains:
Length: 1087 amino acids
Theoretical weight: 121.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical: O00267 (Residues: 1-1087; Coverage: 100%)
Sequence domains:
Negative elongation factor C/D
Chain: W
Molecule details ›
Chain: W
Length: 590 amino acids
Theoretical weight: 66.32 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
Sequence domains: TH1 protein
Length: 590 amino acids
Theoretical weight: 66.32 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
- Canonical: Q8IXH7 (Residues: 1-590; Coverage: 100%)
Sequence domains: TH1 protein
Negative elongation factor B
Chain: V
Molecule details ›
Chain: V
Length: 580 amino acids
Theoretical weight: 65.78 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
Sequence domains: Cofactor of BRCA1 (COBRA1)
Length: 580 amino acids
Theoretical weight: 65.78 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
- Canonical: Q8WX92 (Residues: 1-580; Coverage: 100%)
Sequence domains: Cofactor of BRCA1 (COBRA1)
DNA-directed RNA polymerase subunit
Chain: A
Molecule details ›
Chain: A
Length: 1970 amino acids
Theoretical weight: 217.45 KDa
Source organism: Sus scrofa
UniProt:
Sequence domains:
Length: 1970 amino acids
Theoretical weight: 217.45 KDa
Source organism: Sus scrofa
UniProt:
- Canonical: A0A8D1DPV6 (Residues: 1-1970; Coverage: 100%)
Sequence domains:
DNA-directed RNA polymerase subunit beta
Chain: B
Molecule details ›
Chain: B
Length: 1174 amino acids
Theoretical weight: 134.04 KDa
Source organism: Sus scrofa
UniProt:
Sequence domains:
Length: 1174 amino acids
Theoretical weight: 134.04 KDa
Source organism: Sus scrofa
UniProt:
- Canonical: I3LGP4 (Residues: 127-1300; Coverage: 90%)
Sequence domains:
DNA-directed RNA polymerase RpoA/D/Rpb3-type domain-containing protein
Chain: C
Molecule details ›
Chain: C
Length: 271 amino acids
Theoretical weight: 31 KDa
Source organism: Sus scrofa
UniProt:
Sequence domains:
Length: 271 amino acids
Theoretical weight: 31 KDa
Source organism: Sus scrofa
UniProt:
- Canonical: I3LCH3 (Residues: 1-271; Coverage: 99%)
Sequence domains:
RNA polymerase Rpb4/RPC9 core domain-containing protein
Chain: D
Molecule details ›
Chain: D
Length: 142 amino acids
Theoretical weight: 16.33 KDa
Source organism: Sus scrofa
UniProt:
Length: 142 amino acids
Theoretical weight: 16.33 KDa
Source organism: Sus scrofa
UniProt:
- Canonical: A0A8D0KES4 (Residues: 43-184; Coverage: 77%)
DNA-directed RNA polymerase II subunit E
Chain: E
Molecule details ›
Chain: E
Length: 210 amino acids
Theoretical weight: 24.64 KDa
Source organism: Sus scrofa
UniProt:
Sequence domains:
Length: 210 amino acids
Theoretical weight: 24.64 KDa
Source organism: Sus scrofa
UniProt:
- Canonical: I3LSI7 (Residues: 1-210; Coverage: 100%)
Sequence domains:
DNA-directed RNA polymerases I, II, and III subunit RPABC2
Chain: F
Molecule details ›
Chain: F
Length: 127 amino acids
Theoretical weight: 14.48 KDa
Source organism: Sus scrofa
UniProt:
Sequence domains: RNA polymerase Rpb6
Length: 127 amino acids
Theoretical weight: 14.48 KDa
Source organism: Sus scrofa
UniProt:
- Canonical: A0A4X1VEK9 (Residues: 1-127; Coverage: 100%)
Sequence domains: RNA polymerase Rpb6
DNA-directed RNA polymerase subunit
Chain: G
Molecule details ›
Chain: G
Length: 172 amino acids
Theoretical weight: 19.31 KDa
Source organism: Sus scrofa
UniProt:
Sequence domains:
Length: 172 amino acids
Theoretical weight: 19.31 KDa
Source organism: Sus scrofa
UniProt:
- Canonical: A0A4X1VKG7 (Residues: 1-172; Coverage: 100%)
Sequence domains:
DNA-directed RNA polymerases I, II, and III subunit RPABC3
Chain: H
Molecule details ›
Chain: H
Length: 150 amino acids
Theoretical weight: 17.16 KDa
Source organism: Sus scrofa
UniProt:
Sequence domains: RNA polymerase Rpb8
Length: 150 amino acids
Theoretical weight: 17.16 KDa
Source organism: Sus scrofa
UniProt:
- Canonical: A0A8D1AQR7 (Residues: 1-150; Coverage: 100%)
Sequence domains: RNA polymerase Rpb8
DNA-directed RNA polymerase II subunit RPB9
Chain: I
Molecule details ›
Chain: I
Length: 125 amino acids
Theoretical weight: 14.54 KDa
Source organism: Sus scrofa
UniProt:
Sequence domains:
Length: 125 amino acids
Theoretical weight: 14.54 KDa
Source organism: Sus scrofa
UniProt:
- Canonical: P60899 (Residues: 1-125; Coverage: 100%)
Sequence domains:
DNA-directed RNA polymerases I, II, and III subunit RPABC5
Chain: J
Molecule details ›
Chain: J
Length: 67 amino acids
Theoretical weight: 7.66 KDa
Source organism: Sus scrofa
UniProt:
Sequence domains: RNA polymerases N / 8 kDa subunit
Length: 67 amino acids
Theoretical weight: 7.66 KDa
Source organism: Sus scrofa
UniProt:
- Canonical: A0A8W4F9W9 (Residues: 1-67; Coverage: 100%)
Sequence domains: RNA polymerases N / 8 kDa subunit
DNA-directed RNA polymerase RBP11-like dimerisation domain-containing protein
Chain: K
Molecule details ›
Chain: K
Length: 117 amino acids
Theoretical weight: 13.31 KDa
Source organism: Sus scrofa
UniProt:
Sequence domains: RNA polymerase Rpb3/Rpb11 dimerisation domain
Length: 117 amino acids
Theoretical weight: 13.31 KDa
Source organism: Sus scrofa
UniProt:
- Canonical: A0A8D0TE17 (Residues: 1-117; Coverage: 100%)
Sequence domains: RNA polymerase Rpb3/Rpb11 dimerisation domain
RNA polymerase II, I and III subunit K
Chain: L
Molecule details ›
Chain: L
Length: 58 amino acids
Theoretical weight: 7.02 KDa
Source organism: Sus scrofa
UniProt:
Sequence domains: DNA directed RNA polymerase, 7 kDa subunit
Length: 58 amino acids
Theoretical weight: 7.02 KDa
Source organism: Sus scrofa
UniProt:
- Canonical: A0A4X1TRS6 (Residues: 1-58; Coverage: 100%)
Sequence domains: DNA directed RNA polymerase, 7 kDa subunit
Negative elongation factor A
Chain: U
Transcription elongation factor SPT4
Chain: Y
Molecule details ›
Chain: Y
Length: 117 amino acids
Theoretical weight: 13.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains: Spt4/RpoE2 zinc finger
Length: 117 amino acids
Theoretical weight: 13.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical: P63272 (Residues: 1-117; Coverage: 100%)
Sequence domains: Spt4/RpoE2 zinc finger
Non-template DNA
Chain: N
Molecule details ›
Chain: N
Length: 50 nucleotides
Theoretical weight: 15.41 KDa
Length: 50 nucleotides
Theoretical weight: 15.41 KDa
Template DNA
Chain: T
Molecule details ›
Chain: T
Length: 50 nucleotides
Theoretical weight: 15.4 KDa
Length: 50 nucleotides
Theoretical weight: 15.4 KDa
RNA
Chain: P
Molecule details ›
Chain: P
Length: 22 nucleotides
Theoretical weight: 6.97 KDa
Length: 22 nucleotides
Theoretical weight: 6.97 KDa