Function and Biology Details
Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
- not assigned
Biological process:
- not assigned
Cellular component:
- not assigned
Structure analysis Details
Assembly composition:
hetero 67-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-280372 (preferred)
Entry contents:
58 distinct polypeptide molecules
2 distinct DNA molecules
5 distinct RNA molecules
2 distinct DNA molecules
5 distinct RNA molecules
Macromolecules (65 distinct):
Large ribosomal subunit protein bL21
Chain: 0
Molecule details ›
Chain: 0
Length: 103 amino acids
Theoretical weight: 11.59 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 103 amino acids
Theoretical weight: 11.59 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Large ribosomal subunit protein uL22
Chain: 1
Molecule details ›
Chain: 1
Length: 110 amino acids
Theoretical weight: 12.25 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 110 amino acids
Theoretical weight: 12.25 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Large ribosomal subunit protein uL23
Chain: 2
Molecule details ›
Chain: 2
Length: 100 amino acids
Theoretical weight: 11.22 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 100 amino acids
Theoretical weight: 11.22 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Large ribosomal subunit protein uL24
Chain: 3
Molecule details ›
Chain: 3
Length: 104 amino acids
Theoretical weight: 11.34 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 104 amino acids
Theoretical weight: 11.34 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Large ribosomal subunit protein bL25
Chain: 4
Molecule details ›
Chain: 4
Length: 94 amino acids
Theoretical weight: 10.71 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 94 amino acids
Theoretical weight: 10.71 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Large ribosomal subunit protein uL10
Chain: 9
Molecule details ›
Chain: 9
Length: 165 amino acids
Theoretical weight: 17.74 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 165 amino acids
Theoretical weight: 17.74 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
DNA-directed RNA polymerase subunit beta
Chain: AA
Molecule details ›
Chain: AA
Length: 1342 amino acids
Theoretical weight: 150.82 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 1342 amino acids
Theoretical weight: 150.82 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Transcription antitermination protein RfaH
Chain: AB
Molecule details ›
Chain: AB
Length: 162 amino acids
Theoretical weight: 18.36 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 162 amino acids
Theoretical weight: 18.36 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
DNA-directed RNA polymerase subunit alpha
Chains: AC, AD
Molecule details ›
Chains: AC, AD
Length: 329 amino acids
Theoretical weight: 36.56 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 329 amino acids
Theoretical weight: 36.56 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
DNA-directed RNA polymerase subunit beta'
Chain: AE
Molecule details ›
Chain: AE
Length: 1407 amino acids
Theoretical weight: 155.37 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 1407 amino acids
Theoretical weight: 155.37 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
DNA-directed RNA polymerase subunit omega
Chain: AF
Molecule details ›
Chain: AF
Length: 91 amino acids
Theoretical weight: 10.25 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 91 amino acids
Theoretical weight: 10.25 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Small ribosomal subunit protein bS18
Chain: C
Molecule details ›
Chain: C
Length: 75 amino acids
Theoretical weight: 9.01 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 75 amino acids
Theoretical weight: 9.01 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Small ribosomal subunit protein bS20
Chain: E
Molecule details ›
Chain: E
Length: 87 amino acids
Theoretical weight: 9.71 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 87 amino acids
Theoretical weight: 9.71 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Small ribosomal subunit protein bS21
Chain: F
Molecule details ›
Chain: F
Length: 71 amino acids
Theoretical weight: 8.52 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 71 amino acids
Theoretical weight: 8.52 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Small ribosomal subunit protein uS2
Chain: G
Molecule details ›
Chain: G
Length: 241 amino acids
Theoretical weight: 26.78 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 241 amino acids
Theoretical weight: 26.78 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Small ribosomal subunit protein bS1
Chain: H
Molecule details ›
Chain: H
Length: 557 amino acids
Theoretical weight: 61.24 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 557 amino acids
Theoretical weight: 61.24 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Small ribosomal subunit protein uS3
Chain: I
Molecule details ›
Chain: I
Length: 233 amino acids
Theoretical weight: 26.03 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 233 amino acids
Theoretical weight: 26.03 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Small ribosomal subunit protein uS4
Chain: J
Molecule details ›
Chain: J
Length: 206 amino acids
Theoretical weight: 23.51 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 206 amino acids
Theoretical weight: 23.51 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Small ribosomal subunit protein uS5
Chain: K
Molecule details ›
Chain: K
Length: 167 amino acids
Theoretical weight: 17.63 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 167 amino acids
Theoretical weight: 17.63 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Small ribosomal subunit protein bS6
Chain: L
Molecule details ›
Chain: L
Length: 135 amino acids
Theoretical weight: 15.73 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 135 amino acids
Theoretical weight: 15.73 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Small ribosomal subunit protein uS7
Chain: M
Molecule details ›
Chain: M
Length: 179 amino acids
Theoretical weight: 20.06 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 179 amino acids
Theoretical weight: 20.06 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Small ribosomal subunit protein uS8
Chain: N
Molecule details ›
Chain: N
Length: 130 amino acids
Theoretical weight: 14.15 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 130 amino acids
Theoretical weight: 14.15 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Small ribosomal subunit protein uS9
Chain: O
Molecule details ›
Chain: O
Length: 130 amino acids
Theoretical weight: 14.89 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 130 amino acids
Theoretical weight: 14.89 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Small ribosomal subunit protein uS10
Chain: P
Molecule details ›
Chain: P
Length: 103 amino acids
Theoretical weight: 11.76 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 103 amino acids
Theoretical weight: 11.76 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Small ribosomal subunit protein uS11
Chain: Q
Molecule details ›
Chain: Q
Length: 129 amino acids
Theoretical weight: 13.87 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 129 amino acids
Theoretical weight: 13.87 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Small ribosomal subunit protein uS12
Chain: R
Molecule details ›
Chain: R
Length: 124 amino acids
Theoretical weight: 13.77 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 124 amino acids
Theoretical weight: 13.77 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Small ribosomal subunit protein uS14
Chain: S
Molecule details ›
Chain: S
Length: 101 amino acids
Theoretical weight: 11.61 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 101 amino acids
Theoretical weight: 11.61 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Small ribosomal subunit protein uS15
Chain: T
Molecule details ›
Chain: T
Length: 89 amino acids
Theoretical weight: 10.29 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 89 amino acids
Theoretical weight: 10.29 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Small ribosomal subunit protein bS16
Chain: U
Molecule details ›
Chain: U
Length: 82 amino acids
Theoretical weight: 9.21 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 82 amino acids
Theoretical weight: 9.21 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Small ribosomal subunit protein uS17
Chain: V
Molecule details ›
Chain: V
Length: 84 amino acids
Theoretical weight: 9.72 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 84 amino acids
Theoretical weight: 9.72 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Small ribosomal subunit protein uS19
Chain: W
Molecule details ›
Chain: W
Length: 92 amino acids
Theoretical weight: 10.46 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 92 amino acids
Theoretical weight: 10.46 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Small ribosomal subunit protein uS13
Chain: X
Molecule details ›
Chain: X
Length: 118 amino acids
Theoretical weight: 13.13 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 118 amino acids
Theoretical weight: 13.13 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Large ribosomal subunit protein uL11
Chain: Y
Molecule details ›
Chain: Y
Length: 142 amino acids
Theoretical weight: 14.89 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 142 amino acids
Theoretical weight: 14.89 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Large ribosomal subunit protein bL12
Chain: Z
Molecule details ›
Chain: Z
Length: 121 amino acids
Theoretical weight: 12.31 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 121 amino acids
Theoretical weight: 12.31 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Large ribosomal subunit protein bL27
Chain: b
Molecule details ›
Chain: b
Length: 85 amino acids
Theoretical weight: 9.15 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 85 amino acids
Theoretical weight: 9.15 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Large ribosomal subunit protein bL28
Chain: c
Molecule details ›
Chain: c
Length: 78 amino acids
Theoretical weight: 9.03 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 78 amino acids
Theoretical weight: 9.03 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
50S ribosomal protein L29
Chain: e
Molecule details ›
Chain: e
Length: 63 amino acids
Theoretical weight: 7.29 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 63 amino acids
Theoretical weight: 7.29 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
50S ribosomal protein L30
Chain: f
Molecule details ›
Chain: f
Length: 59 amino acids
Theoretical weight: 6.55 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 59 amino acids
Theoretical weight: 6.55 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Large ribosomal subunit protein bL31
Chain: g
Molecule details ›
Chain: g
Length: 70 amino acids
Theoretical weight: 7.89 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 70 amino acids
Theoretical weight: 7.89 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Large ribosomal subunit protein uL2
Chain: h
Molecule details ›
Chain: h
Length: 273 amino acids
Theoretical weight: 29.92 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 273 amino acids
Theoretical weight: 29.92 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
50S ribosomal protein L32
Chain: i
Molecule details ›
Chain: i
Length: 57 amino acids
Theoretical weight: 6.46 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 57 amino acids
Theoretical weight: 6.46 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Large ribosomal subunit protein uL3
Chain: j
Molecule details ›
Chain: j
Length: 209 amino acids
Theoretical weight: 22.28 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 209 amino acids
Theoretical weight: 22.28 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
50S ribosomal protein L33
Chain: k
Molecule details ›
Chain: k
Length: 55 amino acids
Theoretical weight: 6.39 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 55 amino acids
Theoretical weight: 6.39 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Large ribosomal subunit protein uL4
Chain: l
Molecule details ›
Chain: l
Length: 201 amino acids
Theoretical weight: 22.12 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 201 amino acids
Theoretical weight: 22.12 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Large ribosomal subunit protein bL34
Chain: m
Molecule details ›
Chain: m
Length: 46 amino acids
Theoretical weight: 5.4 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 46 amino acids
Theoretical weight: 5.4 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
50S ribosomal protein L5
Chain: n
Molecule details ›
Chain: n
Length: 179 amino acids
Theoretical weight: 20.33 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 179 amino acids
Theoretical weight: 20.33 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Large ribosomal subunit protein bL35
Chain: o
Molecule details ›
Chain: o
Length: 65 amino acids
Theoretical weight: 7.31 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 65 amino acids
Theoretical weight: 7.31 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Large ribosomal subunit protein uL6
Chain: p
Molecule details ›
Chain: p
Length: 177 amino acids
Theoretical weight: 18.93 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 177 amino acids
Theoretical weight: 18.93 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Large ribosomal subunit protein bL36
Chain: q
Molecule details ›
Chain: q
Length: 38 amino acids
Theoretical weight: 4.38 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 38 amino acids
Theoretical weight: 4.38 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
50S ribosomal protein L9
Chain: r
Molecule details ›
Chain: r
Length: 149 amino acids
Theoretical weight: 15.79 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 149 amino acids
Theoretical weight: 15.79 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
50S ribosomal protein L13
Chain: s
Molecule details ›
Chain: s
Length: 142 amino acids
Theoretical weight: 16.05 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 142 amino acids
Theoretical weight: 16.05 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Large ribosomal subunit protein uL14
Chain: t
Molecule details ›
Chain: t
Length: 123 amino acids
Theoretical weight: 13.57 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 123 amino acids
Theoretical weight: 13.57 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Large ribosomal subunit protein uL15
Chain: u
Molecule details ›
Chain: u
Length: 144 amino acids
Theoretical weight: 15.01 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 144 amino acids
Theoretical weight: 15.01 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Large ribosomal subunit protein uL16
Chain: v
Molecule details ›
Chain: v
Length: 136 amino acids
Theoretical weight: 15.31 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 136 amino acids
Theoretical weight: 15.31 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
50S ribosomal protein L17
Chain: w
Molecule details ›
Chain: w
Length: 127 amino acids
Theoretical weight: 14.39 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 127 amino acids
Theoretical weight: 14.39 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
50S ribosomal protein L18
Chain: x
Molecule details ›
Chain: x
Length: 117 amino acids
Theoretical weight: 12.79 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 117 amino acids
Theoretical weight: 12.79 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
50S ribosomal protein L19
Chain: y
Molecule details ›
Chain: y
Length: 115 amino acids
Theoretical weight: 13.16 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 115 amino acids
Theoretical weight: 13.16 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Large ribosomal subunit protein bL20
Chain: z
Molecule details ›
Chain: z
Length: 118 amino acids
Theoretical weight: 13.53 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Length: 118 amino acids
Theoretical weight: 13.53 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
NT DNA ops
Chain: 5
Molecule details ›
Chain: 5
Length: 38 nucleotides
Theoretical weight: 11.79 KDa
Source organism: synthetic construct
Expression system: synthetic construct
Length: 38 nucleotides
Theoretical weight: 11.79 KDa
Source organism: synthetic construct
Expression system: synthetic construct
T DNA ops
Chain: 6
Molecule details ›
Chain: 6
Length: 38 nucleotides
Theoretical weight: 11.53 KDa
Source organism: synthetic construct
Expression system: synthetic construct
Length: 38 nucleotides
Theoretical weight: 11.53 KDa
Source organism: synthetic construct
Expression system: synthetic construct
mRNA with 21 nt long spacer
Chain: 7
Molecule details ›
Chain: 7
Length: 38 nucleotides
Theoretical weight: 11.99 KDa
Source organism: synthetic construct
Expression system: synthetic construct
Length: 38 nucleotides
Theoretical weight: 11.99 KDa
Source organism: synthetic construct
Expression system: synthetic construct
E-site and P-site tRNA (fMet)
Chains: A, B
Molecule details ›
Chains: A, B
Length: 76 nucleotides
Theoretical weight: 24.5 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Sequence domains: tRNA
Length: 76 nucleotides
Theoretical weight: 24.5 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Sequence domains: tRNA
16S ribosomal RNA
Chain: D
Molecule details ›
Chain: D
Length: 1542 nucleotides
Theoretical weight: 499.69 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Sequence domains: Bacterial small subunit ribosomal RNA
Length: 1542 nucleotides
Theoretical weight: 499.69 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Sequence domains: Bacterial small subunit ribosomal RNA
23S ribosomal RNA
Chain: a
Molecule details ›
Chain: a
Length: 2904 nucleotides
Theoretical weight: 941.64 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Sequence domains: Bacterial large subunit ribosomal RNA
Length: 2904 nucleotides
Theoretical weight: 941.64 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Sequence domains: Bacterial large subunit ribosomal RNA
5S ribosomal RNA
Chain: d
Molecule details ›
Chain: d
Length: 120 nucleotides
Theoretical weight: 38.79 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Sequence domains: 5S ribosomal RNA
Length: 120 nucleotides
Theoretical weight: 38.79 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Sequence domains: 5S ribosomal RNA