8wpu

Electron Microscopy
3.1Å resolution

Human calcium-sensing receptor(CaSR) bound to cinacalcet in complex with Gq protein

Released:

Function and Biology Details

Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-272302 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (6 distinct):
Extracellular calcium-sensing receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 1076 amino acids
Theoretical weight: 120.64 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P41180 (Residues: 20-907; Coverage: 84%)
Gene names: CASR, GPRC2A, PCAR1
Sequence domains:
Guanine nucleotide-binding protein G(i) subunit alpha-2; Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: G
Molecule details ›
Chain: G
Length: 246 amino acids
Theoretical weight: 28.08 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P04899 (Residues: 1-182, 310-310, 320-320, 348-349; Coverage: 20%)
  • Canonical: P63092 (Residues: 205-346, 348-357, 359-386, 389-394; Coverage: 45%)
Gene names: GNAI2, GNAI2B, GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: C
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: D
ScFv16 Chain: E

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-37724
Expression system: Spodoptera frugiperda