EMPIAR-10325

Raw FIB SEM images of a parasitophorous vacuole containing Toxoplasma gondii ∆CAP parasites, complemented with CAP.

Entry authors:
Hunt A , Russell MRG , Wagener J , Kent R, Carmeille R, Peddie CJ, Collinson L, Heaslip A, Ward GE, Treeck M 
Publication:
Differential requirements for cyclase-associated protein (CAP) in actin-dependent processes of Toxoplasma gondii
Hunt A , Russell MRG , Wagener J , Kent R , Carmeille R, Peddie CJ, Collinson L, Heaslip A, Ward GE , Treeck M 
Elife 8 (2019)
PMID: 31577230
Experiment type:
FIB-SEM
Deposited:
2019-09-30
Released:
2019-10-07
Last modified:
2019-10-07
Dataset size:
605.2 GB
Experimental metadata:
Contains:
micrographs
Image not available

Image set

1. Raw FIB SEM images of a parasitophorous vacuole containing Toxoplasma gondii ∆CAP parasites, complemented with CAP.
Category:micrographs - single frame
Image format:TIFF
No. of images or tilt series:4529
Image size:(8384, 8384)
Pixel type:UNSIGNED 16 BIT INTEGER
Pixel spacing:(50 Å, 50 Å)
Details:
.Image acquisition;
5 nm voxels: xy pixel set to 5 nm, FIB mill every 5 nm.
SEM: 1.5 kV with 1 nA current, 10 μs dwell time.
EsB detector grid voltage: 1200 V.
Ion beam milling: 30 kV, 700 pA.
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