› 1a00

X-ray diffraction
2Å resolution

HEMOGLOBIN (VAL BETA1 MET, TRP BETA37 TYR) MUTANT

Released:
DOI: 10.2210/pdb1a00/pdb
PDB entry 1a00 coloured by chain, front view.
PDB entry 1a00 coloured by chain, side view.
PDB entry 1a00 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
High-resolution crystal structures of human hemoglobin with mutations at tryptophan 37beta: structural basis for a high-affinity T-state,.
Kavanaugh JS, Weydert JA, Rogers PH, Arnone A
Biochemistry 37 4358-73 (1998)
PMID: 9521756

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159519 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Hemoglobin subunit alpha Chains: A, C
Molecule details ›
Chains: A, C
Length: 141 amino acids
Theoretical weight: 15.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P69905 (Residues: 2-142; Coverage: 99%)
Gene names: HBA1, HBA2
Sequence domains: Globin
Structure domains: Globins
Hemoglobin subunit beta Chains: B, D
Molecule details ›
Chains: B, D
Length: 146 amino acids
Theoretical weight: 15.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P68871 (Residues: 2-147; Coverage: 99%)
Gene name: HBB
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

1 bound ligand:
Ligand HEM 4 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P212121
Unit cell:
a: 84.1Å b: 112Å c: 63.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.169 0.223
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Hydrophobicity: is LogP(o/w) more than the sum of its parts?
Eugene Kellogg et al. (2000)
3 more

9 mentions without citation

PDBj Mine: design and implementation of relational database interface for Protein Data Bank Japan.
Kinjo et al. (2010)
8 more