› 1ary

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURES OF CYANIDE-AND TRIIODIDE-BOUND FORMS OF ARTHROMYCES RAMOSUS PEROXIDASE AT DIFFERENT PH VALUES. PERTURBATIONS OF ACTIVE SITE RESIDUES AND THEIR IMPLICATION IN ENZYME CATALYSIS

Released:
DOI: 10.2210/pdb1ary/pdb
PDB entry 1ary coloured by chain, front view.
PDB entry 1ary coloured by chain, side view.
PDB entry 1ary coloured by chain, top view.
Source organism: Agaricales sp. 'Arthromyces ramosus'
Primary publication:
Crystal structures of cyanide- and triiodide-bound forms of Arthromyces ramosus peroxidase at different pH values. Perturbations of active site residues and their implication in enzyme catalysis.
Fukuyama K, Kunishima N, Amada F, Kubota T, Matsubara H
J Biol Chem 270 21884-92 (1995)
PMID: 7665612

Function and Biology Details

Reaction catalysed: 
2 phenolic donor + H(2)O(2) = 2 phenoxyl radical of the donor + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151200 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Peroxidase Chain: A
Molecule details ›
Chain: A
Length: 344 amino acids
Theoretical weight: 35.72 KDa
Source organism: Agaricales sp. 'Arthromyces ramosus'
UniProt:
  • Canonical: P28313 (Residues: 21-364; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand CA 2 x CA
Ligand IOD 7 x IOD
Ligand K 1 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P42212
Unit cell:
a: 74.57Å b: 74.57Å c: 117.47Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.168 not available

Quick links

Citations

2 review citations

Suicide inactivation of peroxidases and the challenge of engineering more robust enzymes.
Valderrama et al. (2002)
1 more