› 1b10

Solution NMR

SOLUTION NMR STRUCTURE OF RECOMBINANT SYRIAN HAMSTER PRION PROTEIN RPRP(90-231) , 25 STRUCTURES

Released:
DOI: 10.2210/pdb1b10/pdb
PDB entry 1b10 coloured by chain, ensemble of 25 models, front view.
PDB entry 1b10 coloured by chain, ensemble of 25 models, side view.
PDB entry 1b10 coloured by chain, ensemble of 25 models, top view.
Source organism: Mesocricetus auratus
Primary publication:
Solution structure of a 142-residue recombinant prion protein corresponding to the infectious fragment of the scrapie isoform.
James TL, Liu H, Ulyanov NB, Farr-Jones S, Zhang H, Donne DG, Kaneko K, Groth D, Mehlhorn I, Prusiner SB, Cohen FE
Proc Natl Acad Sci U S A 94 10086-91 (1997)
PMID: 9294167

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137558 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Major prion protein Chain: A
Molecule details ›
Chain: A
Length: 142 amino acids
Theoretical weight: 16.26 KDa
Source organism: Mesocricetus auratus
Expression system: Escherichia coli
UniProt:
  • Canonical: P04273 (Residues: 90-231; Coverage: 61%)
Gene names: PRNP, PRP
Sequence domains: Prion/Doppel alpha-helical domain
Structure domains: Prion/Doppel protein, beta-ribbon domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: NMR-RESTRAINED SIMULATED ANNEALING, MOLECULAR DYNAMICS, AND ENERGY MINIMIZATION.
Expression system: Escherichia coli

Quick links

Citations

55 review citations

Prions.
Prusiner SB. (1998)
54 more

55 mentions without citation

A structural overview of the vertebrate prion proteins.
Pastore et al. (2007)
54 more