› 1bhn

X-ray diffraction
2.4Å resolution

NUCLEOSIDE DIPHOSPHATE KINASE ISOFORM A FROM BOVINE RETINA

Released:
DOI: 10.2210/pdb1bhn/pdb
PDB entry 1bhn coloured by chain, front view.
PDB entry 1bhn coloured by chain, side view.
PDB entry 1bhn coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
The three-dimensional structures of two isoforms of nucleoside diphosphate kinase from bovine retina.
Ladner JE, Abdulaev NG, Kakuev DL, Tordová M, Ridge KD, Gilliland GL
Acta Crystallogr D Biol Crystallogr 55 1127-35 (1999)
PMID: 10329774

Function and Biology Details

Reaction catalysed: 
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156473 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleoside diphosphate kinase A 1 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 152 amino acids
Theoretical weight: 17.28 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P52174 (Residues: 1-152; Coverage: 100%)
Gene name: NME1-1
Sequence domains: Nucleoside diphosphate kinase
Structure domains: Nucleoside diphosphate kinase-like domain

Ligands and Environments

2 bound ligands:
Ligand 35G 6 x 35G
Ligand GDP 6 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SIEMENS
Spacegroup: P212121
Unit cell:
a: 89.88Å b: 92.11Å c: 131.63Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 not available not available
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Three-dimensional structure of nucleoside diphosphate kinase.
Janin et al. (2000)
6 other articles

2 mentions without citation

Molecular mechanism of distinct salt-dependent enzyme activity of two halophilic nucleoside diphosphate kinases.
Yamamura et al. (2009)
1 more