› 1c8u

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF THE E.COLI THIOESTERASE II, A HOMOLOGUE OF THE HUMAN NEF-BINDING ENZYME

Released:
DOI: 10.2210/pdb1c8u/pdb
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PDB entry 1c8u coloured by chain, front view.
PDB entry 1c8u coloured by chain, side view.
PDB entry 1c8u coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of the Escherichia coli thioesterase II, a homolog of the human Nef binding enzyme.
Li J, Derewenda U, Dauter Z, Smith S, Derewenda ZS
Nat Struct Biol 7 555-9 (2000)
PMID: 10876240

Function and Biology Details

Reaction catalysed: 
Acyl-CoA + H(2)O = CoA + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142834 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acyl-CoA thioesterase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 285 amino acids
Theoretical weight: 31.87 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AGG2 (Residues: 2-286; Coverage: 100%)
Gene names: JW0442, b0452, tesB
Sequence domains: Acyl-CoA thioesterase
Structure domains: Hotdog Thioesterase

Ligands and Environments

1 bound ligand:
Ligand LDA 2 x LDA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: C2221
Unit cell:
a: 95.902Å b: 119.805Å c: 165.479Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.218 0.248
Expression system: Escherichia coli BL21(DE3)

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Citations

6 review citations

GDSL family of serine esterases/lipases.
Akoh et al. (2004)
5 more

17 mentions without citation

Thioesterases: a new perspective based on their primary and tertiary structures.
Cantu et al. (2010)
16 more

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