› 1d3c

X-ray diffraction
1.78Å resolution

MICHAELIS COMPLEX OF BACILLUS CIRCULANS STRAIN 251 CYCLODEXTRIN GLYCOSYLTRANSFERASE WITH GAMMA-CYCLODEXTRIN

Released:
DOI: 10.2210/pdb1d3c/pdb
PDB entry 1d3c coloured by chain, front view.
PDB entry 1d3c coloured by chain, side view.
PDB entry 1d3c coloured by chain, top view.
Source organism: Niallia circulans
Primary publication:
The cyclization mechanism of cyclodextrin glycosyltransferase (CGTase) as revealed by a gamma-cyclodextrin-CGTase complex at 1.8-A resolution.
Uitdehaag JC, Kalk KH, van Der Veen BA, Dijkhuizen L, Dijkstra BW
J Biol Chem 274 34868-76 (1999)
PMID: 10574960

Function and Biology Details

Reaction catalysed: 
Cyclizes part of a (1->4)-alpha-D-glucan chain by formation of a (1->4)-alpha-D-glucosidic bond
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155054 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Cyclomaltodextrin glucanotransferase Chain: A
Molecule details ›
Chain: A
Length: 686 amino acids
Theoretical weight: 74.57 KDa
Source organism: Niallia circulans
Expression system: Bacillus subtilis
UniProt:
  • Canonical: P43379 (Residues: 28-713; Coverage: 100%)
Gene name: cgt
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
2 bound ligands:
Ligand CA 3 x CA
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P212121
Unit cell:
a: 117.791Å b: 109.532Å c: 65.223Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.22 0.258
Expression system: Bacillus subtilis

Quick links

Citations

10 review citations

Relationship of sequence and structure to specificity in the alpha-amylase family of enzymes.
MacGregor et al. (2001)
9 more

12 mentions without citation

Functional map and domain structure of MET, the product of the c-met protooncogene and receptor for hepatocyte growth factor/scatter factor.
Gherardi et al. (2003)
11 more