› 1ded

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF ALKALOPHILIC ASPARAGINE 233-REPLACED CYCLODEXTRIN GLUCANOTRANSFERASE COMPLEXED WITH AN INHIBITOR, ACARBOSE, AT 2.0 A RESOLUTION

Released:
DOI: 10.2210/pdb1ded/pdb
PDB entry 1ded coloured by chain, front view.
PDB entry 1ded coloured by chain, side view.
PDB entry 1ded coloured by chain, top view.
Source organism: Bacillus sp. 1011
Primary publication:
Crystal structure of alkalophilic asparagine 233-replaced cyclodextrin glucanotransferase complexed with an inhibitor, acarbose, at 2.0 A resolution.
Ishii N, Haga K, Yamane K, Harata K
J Biochem 127 383-91 (2000)
PMID: 10731709

Function and Biology Details

Reaction catalysed: 
Cyclizes part of a (1->4)-alpha-D-glucan chain by formation of a (1->4)-alpha-D-glucosidic bond
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138719 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Cyclomaltodextrin glucanotransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 686 amino acids
Theoretical weight: 75.21 KDa
Source organism: Bacillus sp. 1011
Expression system: Escherichia coli
UniProt:
  • Canonical: P05618 (Residues: 28-713; Coverage: 100%)
Gene name: cgt
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GLC, AC1
Ligand BGC 3 x BGC
Ligand GLC 3 x GLC
Ligand AC1 3 x AC1
1 bound ligand:
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR571
Spacegroup: P1
Unit cell:
a: 64.93Å b: 73.83Å c: 79.05Å
α: 85.1° β: 105.4° γ: 100.5°
R-values:
R R work R free
0.163 0.163 0.222
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Relationship of sequence and structure to specificity in the alpha-amylase family of enzymes.
MacGregor et al. (2001)
1 more

1 mention without citation

A new definition and properties of the similarity value between two protein structures.
Saberi Fathi SM. (2016)