› 1eo7

X-ray diffraction
2.48Å resolution

BACILLUS CIRCULANS STRAIN 251 CYCLODEXTRIN GLYCOSYLTRANSFERASE IN COMPLEX WITH MALTOHEXAOSE

Released:
DOI: 10.2210/pdb1eo7/pdb
PDB entry 1eo7 coloured by chain, front view.
PDB entry 1eo7 coloured by chain, side view.
PDB entry 1eo7 coloured by chain, top view.
Source organism: Niallia circulans
Primary publication:
Structures of maltohexaose and maltoheptaose bound at the donor sites of cyclodextrin glycosyltransferase give insight into the mechanisms of transglycosylation activity and cyclodextrin size specificity.
Uitdehaag JC, van Alebeek GJ, van Der Veen BA, Dijkhuizen L, Dijkstra BW
Biochemistry 39 7772-80 (2000)
PMID: 10869182

Function and Biology Details

Reaction catalysed: 
Cyclizes part of a (1->4)-alpha-D-glucan chain by formation of a (1->4)-alpha-D-glucosidic bond
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155054 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Cyclomaltodextrin glucanotransferase Chain: A
Molecule details ›
Chain: A
Length: 686 amino acids
Theoretical weight: 74.46 KDa
Source organism: Niallia circulans
Expression system: Bacillus subtilis
UniProt:
  • Canonical: P43379 (Residues: 28-713; Coverage: 100%)
Gene name: cgt
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
1 bound ligand:
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P212121
Unit cell:
a: 111.648Å b: 109.022Å c: 64.677Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.228 0.296
Expression system: Bacillus subtilis

Quick links

Citations

7 review citations

gamma-Cyclodextrin: a review on enzymatic production and applications.
Li et al. (2007)
6 more