› 1epy

X-ray diffraction
1.85Å resolution

T4 LYSOZYME MUTANT, T21H/C54T/C97A/Q141H/T142H

Released:
DOI: 10.2210/pdb1epy/pdb
PDB entry 1epy coloured by chain, front view.
PDB entry 1epy coloured by chain, side view.
PDB entry 1epy coloured by chain, top view.
Source organism: Escherichia virus T4
Primary publication:
Use of a non-rigid region in T4 lysozyme to design an adaptable metal-binding site.
Wray JW, Baase WA, Ostheimer GJ, Zhang XJ, Matthews BW
Protein Eng 13 313-21 (2000)
PMID: 10835104

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133020 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endolysin Chain: A
Molecule details ›
Chain: A
Length: 164 amino acids
Theoretical weight: 18.71 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli
UniProt:
  • Canonical: P00720 (Residues: 1-164; Coverage: 100%)
Gene name: E
Sequence domains: Phage lysozyme
Structure domains: Lysozyme

Ligands and Environments

3 bound ligands:
Ligand SO4 1 x SO4
Ligand CL 1 x CL
Ligand CO 1 x CO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P3221
Unit cell:
a: 62.385Å b: 62.385Å c: 97.241Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 not available not available
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Lessons from the lysozyme of phage T4.
Baase et al. (2010)
1 more