› 1exw

X-ray diffraction
2.4Å resolution

CRYSTAL STRUCTURE OF PALMITOYL PROTEIN THIOESTERASE 1 COMPLEXED WITH HEXADECYLSULFONYL FLUORIDE

Released:
DOI: 10.2210/pdb1exw/pdb
PDB entry 1exw coloured by chain, front view.
PDB entry 1exw coloured by chain, side view.
PDB entry 1exw coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structural basis for the insensitivity of a serine enzyme (palmitoyl-protein thioesterase) to phenylmethylsulfonyl fluoride.
Das AK, Bellizzi JJ, Tandel S, Biehl E, Clardy J, Hofmann SL
J Biol Chem 275 23847-51 (2000)
PMID: 10801859

Function and Biology Details

Reaction catalysed: 
Palmitoyl-[protein] + H(2)O = palmitate + [protein]
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155293 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Palmitoyl-protein thioesterase 1 Chain: A
Molecule details ›
Chain: A
Length: 279 amino acids
Theoretical weight: 31.45 KDa
Source organism: Bos taurus
Expression system: unidentified baculovirus
UniProt:
  • Canonical: P45478 (Residues: 28-306; Coverage: 100%)
Gene names: CLN1, PPT, PPT1
Sequence domains: Palmitoyl protein thioesterase
Structure domains: alpha/beta hydrolase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand NAG 2 x NAG
Ligand HDS 1 x HDS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P4122
Unit cell:
a: 69.06Å b: 69.06Å c: 128.03Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.243 0.243 0.288
Expression system: unidentified baculovirus

Quick links

Citations

6 review citations

The metabolic serine hydrolases and their functions in mammalian physiology and disease.
Long et al. (2011)
5 more

7 mentions without citation

Thioesterases: a new perspective based on their primary and tertiary structures.
Cantu et al. (2010)
6 more