› 1fa3

Solution NMR

SOLUTION STRUCTURE OF MNEI, A SWEET PROTEIN

Released:
DOI: 10.2210/pdb1fa3/pdb
PDB entry 1fa3 coloured by chain, ensemble of 20 models, front view.
PDB entry 1fa3 coloured by chain, ensemble of 20 models, side view.
PDB entry 1fa3 coloured by chain, ensemble of 20 models, top view.
Source organism: Dioscoreophyllum cumminsii
Primary publication:
Solution structure of a sweet protein: NMR study of MNEI, a single chain monellin.
Spadaccini R, Crescenzi O, Tancredi T, De Casamassimi N, Saviano G, Scognamiglio R, Di Donato A, Temussi PA
J Mol Biol 305 505-14 (2001)
PMID: 11152608

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136329 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Monellin chain B Chain: A
Molecule details ›
Chain: A
Length: 96 amino acids
Theoretical weight: 11.29 KDa
Source organism: Dioscoreophyllum cumminsii
Expression system: Escherichia coli
UniProt:
  • Canonical: P02882 (Residues: 1-50; Coverage: 100%)
Sequence domains: Monellin
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: torsion angle dynamics energy restrained minimization
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Developments in biotechnological production of sweet proteins.
Masuda et al. (2006)
2 more

5 mentions without citation

Probing the surface of a sweet protein: NMR study of MNEI with a paramagnetic probe.
Niccolai et al. (2001)
4 more