› 1fds

X-ray diffraction
1.7Å resolution

HUMAN 17-BETA-HYDROXYSTEROID-DEHYDROGENASE TYPE 1 COMPLEXED WITH 17-BETA-ESTRADIOL

Released:
DOI: 10.2210/pdb1fds/pdb
PDB entry 1fds coloured by chain, front view.
PDB entry 1fds coloured by chain, side view.
PDB entry 1fds coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structure of a complex of human 17beta-hydroxysteroid dehydrogenase with estradiol and NADP+ identifies two principal targets for the design of inhibitors.
Breton R, Housset D, Mazza C, Fontecilla-Camps JC
Structure 4 905-15 (1996)
PMID: 8805577

Function and Biology Details

Reactions catalysed: 
Testosterone + NAD(P)(+) = androst-4-ene-3,17-dione + NAD(P)H
17-beta-estradiol + NAD(P)(+) = estrone + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-537490 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
17-beta-hydroxysteroid dehydrogenase type 1 Chain: A
Molecule details ›
Chain: A
Length: 327 amino acids
Theoretical weight: 34.97 KDa
Source organism: Homo sapiens
Expression system: unidentified baculovirus
UniProt:
  • Canonical: P14061 (Residues: 2-328; Coverage: 100%)
Gene names: E17KSR, EDH17B1, EDH17B2, EDHB17, HSD17B1, SDR28C1
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:
Ligand EST 1 x EST
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LURE BEAMLINE DW32
Spacegroup: C2
Unit cell:
a: 122.33Å b: 44.84Å c: 60.96Å
α: 90° β: 98.84° γ: 90°
R-values:
R R work R free
0.178 0.178 0.203
Expression system: unidentified baculovirus

Quick links

Citations

14 review citations

Molecular endocrinology of hydroxysteroid dehydrogenases.
Penning TM. (1997)
13 more

12 mentions without citation

The structural biology of oestrogen metabolism.
Thomas et al. (2013)
11 more