› 1fsj

X-ray diffraction
1.8Å resolution

CRYSTAL STRUCTURE OF THE E9 DNASE DOMAIN

Released:
DOI: 10.2210/pdb1fsj/pdb
PDB entry 1fsj coloured by chain, front view.
PDB entry 1fsj coloured by chain, side view.
PDB entry 1fsj coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Kuhlmann UC, Pommer AJ, Moore GR, James R, Kleanthous C, Hemmings AM

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140794 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Colicin-E9 Chains: B, C, D, E
Molecule details ›
Chains: B, C, D, E
Length: 134 amino acids
Theoretical weight: 15.12 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P09883 (Residues: 450-582; Coverage: 23%)
Gene names: cei, col
Sequence domains: Colicin/Pyocin-S2, DNase domain
Structure domains: Colicin/pyocin, DNase domain

Ligands and Environments

2 bound ligands:
Ligand PO4 4 x PO4
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P212121
Unit cell:
a: 57.12Å b: 82.96Å c: 113.66Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.189 0.222
Expression system: Escherichia coli

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