1fw7

Solution NMR

NMR STRUCTURE OF 15N-LABELED BARNASE

Released:
Source organism: Bacillus amyloliquefaciens
Entry authors: Reibarkh MY, Vasilieva LI, Schulga AA, Kirpichnikov MP, Arseniev AS

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132802 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease Chain: A
Molecule details ›
Chain: A
Length: 110 amino acids
Theoretical weight: 12.4 KDa
Source organism: Bacillus amyloliquefaciens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00648 (Residues: 48-157; Coverage: 89%)
Sequence domains: ribonuclease
Structure domains: Microbial ribonucleases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: Torsion angle dynamics (DYANA). Newton-Raphson minimization of conformational energy with experimental constraints (FANTOM)
Expression system: Escherichia coli