› 1gph

X-ray diffraction
3Å resolution

STRUCTURE OF THE ALLOSTERIC REGULATORY ENZYME OF PURINE BIOSYNTHESIS

Released:
DOI: 10.2210/pdb1gph/pdb
Model geometry
Fit model/data
Data not deposited
PDB entry 1gph coloured by chain, front view.
PDB entry 1gph coloured by chain, side view.
PDB entry 1gph coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Structure of the allosteric regulatory enzyme of purine biosynthesis.
Smith JL, Zaluzec EJ, Wery JP, Niu L, Switzer RL, Zalkin H, Satow Y
Science 264 1427-33 (1994)
PMID: 8197456

Function and Biology Details

Reaction catalysed: 
5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132565 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amidophosphoribosyltransferase Chains: 1, 2, 3, 4
Molecule details ›
Chains: 1, 2, 3, 4
Length: 465 amino acids
Theoretical weight: 50.51 KDa
Source organism: Bacillus subtilis
Expression system: Not provided
UniProt:
  • Canonical: P00497 (Residues: 12-476; Coverage: 98%)
Gene names: BSU06490, purF
Sequence domains: Glutamine amidotransferase domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SF4 4 x SF4
Ligand AMP 8 x AMP
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 158.8Å b: 75.7Å c: 94.1Å
α: 90° β: 91.4° γ: 90°
R-values:
R R work R free
0.182 0.182 not available
Expression system: Not provided

Quick links

Citations

26 review citations

Structure, function, and formation of biological iron-sulfur clusters.
Johnson et al. (2005)
25 more

5 mentions without citation

The Blueprint of a Minimal Cell: MiniBacillus.
Reuß et al. (2016)
4 more