› 1gxw

X-ray diffraction
2.18Å resolution

the 2.2 A resolution structure of thermolysin crystallized in presence of potassium thiocyanate

Released:
DOI: 10.2210/pdb1gxw/pdb
PDB entry 1gxw coloured by chain, front view.
PDB entry 1gxw coloured by chain, side view.
PDB entry 1gxw coloured by chain, top view.
Source organism: Bacillus thermoproteolyticus
Primary publication:
The 2.2 A resolution structure of thermolysin (TLN) crystallized in the presence of potassium thiocyanate.
Gaucher JF, Selkti M, Prangé T, Tomas A
Acta Crystallogr D Biol Crystallogr 58 2198-200 (2002)
PMID: 12454500

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: -|-Leu > -|-Phe.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133580 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thermolysin Chain: A
Molecule details ›
Chain: A
Length: 316 amino acids
Theoretical weight: 34.36 KDa
Source organism: Bacillus thermoproteolyticus
UniProt:
  • Canonical: P00800 (Residues: 233-548; Coverage: 61%)
Gene name: npr
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand VAL 1 x VAL
Ligand LYS 1 x LYS
Ligand CA 4 x CA
Ligand ZN 1 x ZN
Ligand SCN 1 x SCN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LURE BEAMLINE DW21B
Spacegroup: P6122
Unit cell:
a: 93.17Å b: 93.17Å c: 130.63Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.163 0.163 0.215

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