› 1i45

X-ray diffraction
1.8Å resolution

YEAST TRIOSEPHOSPHATE ISOMERASE (MUTANT)

Released:
DOI: 10.2210/pdb1i45/pdb
PDB entry 1i45 coloured by chain, front view.
PDB entry 1i45 coloured by chain, side view.
PDB entry 1i45 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Solution-state NMR investigations of triosephosphate isomerase active site loop motion: ligand release in relation to active site loop dynamics.
Rozovsky S, Jogl G, Tong L, McDermott AE
J Mol Biol 310 271-80 (2001)
PMID: 11419952

Function and Biology Details

Reaction catalysed: 
D-glyceraldehyde 3-phosphate = glycerone phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133703 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Triosephosphate isomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 248 amino acids
Theoretical weight: 26.78 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P00942 (Residues: 2-248; Coverage: 100%)
Gene names: TPI1, YD9609.05C, YDR050C
Sequence domains: Triosephosphate isomerase
Structure domains: Aldolase class I

Ligands and Environments

No bound ligands
1 modified residue:
Ligand FTR 2 x FTR

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 60.601Å b: 97.058Å c: 49.245Å
α: 90° β: 91.7° γ: 90°
R-values:
R R work R free
0.185 0.175 0.208
Expression system: Escherichia coli

Quick links

Citations

11 review citations

A new spin on protein dynamics.
Columbus et al. (2002)
10 more

2 mentions without citation

Rapid catalytic template searching as an enzyme function prediction procedure.
Nilmeier et al. (2013)
1 more