› 1ilu

X-ray diffraction
2.3Å resolution

X-RAY CRYSTAL STRUCTURE THE TWO SITE-SPECIFIC MUTANTS ILE7SER AND PHE110SER OF AZURIN FROM PSEUDOMONAS AERUGINOSA

Released:
DOI: 10.2210/pdb1ilu/pdb
PDB entry 1ilu coloured by chain, front view.
PDB entry 1ilu coloured by chain, side view.
PDB entry 1ilu coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
X-ray crystal structure of the two site-specific mutants Ile7Ser and Phe110Ser of azurin from Pseudomonas aeruginosa.
Hammann C, Messerschmidt A, Huber R, Nar H, Gilardi G, Canters GW
J Mol Biol 255 362-6 (1996)
PMID: 8568881

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132372 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Azurin Chains: A, B, C, D, E, F, G, H, I, K, L, M
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, K, L, M
Length: 128 amino acids
Theoretical weight: 13.9 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P00282 (Residues: 21-148; Coverage: 100%)
Gene names: PA4922, azu
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

1 bound ligand:
Ligand CU 12 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 41.4Å b: 170.1Å c: 99.7Å
α: 90° β: 91.3° γ: 90°
R-values:
R R work R free
0.171 0.171 not available
Expression system: Escherichia coli K-12

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Citations

14 citation in other articles

Decomposition of protein tryptophan fluorescence spectra into log-normal components. III. Correlation between fluorescence and microenvironment parameters of individual tryptophan residues.
Reshetnyak et al. (2001)
13 more