› 1ioo

X-ray diffraction
1.55Å resolution

CRYSTAL STRUCTURE OF NICOTIANA ALATA GEMETOPHYTIC SELF-INCOMPATIBILITY ASSOCIATED SF11-RNASE

Released:
DOI: 10.2210/pdb1ioo/pdb
PDB entry 1ioo coloured by chain, front view.
PDB entry 1ioo coloured by chain, side view.
PDB entry 1ioo coloured by chain, top view.
Source organism: Nicotiana alata
Primary publication:
The 1.55 A resolution structure of Nicotiana alata S(F11)-RNase associated with gametophytic self-incompatibility.
Ida K, Norioka S, Yamamoto M, Kumasaka T, Yamashita E, Newbigin E, Clarke AE, Sakiyama F, Sato M
J Mol Biol 314 103-12 (2001)
PMID: 11724536

Function and Biology Details

Reaction catalysed: 
(1a) RNA = an (RNA fragment)-3'-nucleoside-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA fragment)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181958 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Ribonuclease S-F11 Chains: A, B
Molecule details ›
Chains: A, B
Length: 196 amino acids
Theoretical weight: 23.13 KDa
Source organism: Nicotiana alata
UniProt:
  • Canonical: Q7SID5 (Residues: 1-196; Coverage: 100%)
Sequence domains: Ribonuclease T2 family
Structure domains: Ribonuclease T2-like

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, XYP, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, XYP, MAN
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL45XU
Spacegroup: P21
Unit cell:
a: 65.869Å b: 44.731Å c: 64.36Å
α: 90° β: 90.27° γ: 90°
R-values:
R R work R free
0.185 0.185 0.215

Quick links

Citations

11 review citations

Self-incompatibility in plants.
Takayama et al. (2005)
10 more

4 mentions without citation

Evolutionary patterns at the RNase based gametophytic self - incompatibility system in two divergent Rosaceae groups (Maloideae and Prunus).
Vieira et al. (2010)
3 more