› 1iqp

X-ray diffraction
2.8Å resolution

Crystal Structure of the Clamp Loader Small Subunit from Pyrococcus furiosus

Released:
DOI: 10.2210/pdb1iqp/pdb
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Source organism: Pyrococcus furiosus
Primary publication:
Atomic structure of the clamp loader small subunit from Pyrococcus furiosus.
Oyama T, Ishino Y, Cann IK, Ishino S, Morikawa K
Mol Cell 8 455-63 (2001)
PMID: 11545747

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186495 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Replication factor C small subunit, 2nd part Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 327 amino acids
Theoretical weight: 37.46 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8U4J3 (Residues: 544-570, 571-581, 582-852; Coverage: 36%)
Gene names: PF0093, rfcS
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ADP 4 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6B
Spacegroup: P212121
Unit cell:
a: 98.361Å b: 105.608Å c: 316.898Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.224 0.277
Expression system: Escherichia coli BL21(DE3)

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Citations

15 review citations

The replication clamp-loading machine at work in the three domains of life.
Indiani et al. (2006)
14 more

8 mentions without citation

DNA replication in the archaea.
Barry et al. (2006)
7 more

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