› 1irm

X-ray diffraction
2.55Å resolution

Crystal structure of apo heme oxygenase-1

Released:
DOI: 10.2210/pdb1irm/pdb
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PDB entry 1irm coloured by chain, front view.
PDB entry 1irm coloured by chain, side view.
PDB entry 1irm coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Crystal structure of rat apo-heme oxygenase-1 (HO-1): mechanism of heme binding in HO-1 inferred from structural comparison of the apo and heme complex forms.
Sugishima M, Sakamoto H, Kakuta Y, Omata Y, Hayashi S, Noguchi M, Fukuyama K
Biochemistry 41 7293-300 (2002)
PMID: 12044160

Function and Biology Details

Reaction catalysed: 
Protoheme + 3 [reduced NADPH--hemoprotein reductase] + 3 O(2) = biliverdin + Fe(2+) + CO + 3 [oxidized NADPH--hemoprotein reductase] + 3 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139216 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heme oxygenase 1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 267 amino acids
Theoretical weight: 30.61 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P06762 (Residues: 1-267; Coverage: 92%)
Gene name: Hmox1
Sequence domains: Heme oxygenase
Structure domains: Heme oxygenase-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P32
Unit cell:
a: 70.21Å b: 70.21Å c: 140.46Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.204 0.202 0.307
Expression system: Escherichia coli

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Citations

7 review citations

Heme oxygenase and heme degradation.
Kikuchi et al. (2005)
6 more

3 mentions without citation

Characterization of multi-locus imprinting disturbances and underlying genetic defects in patients with chromosome 11p15.5 related imprinting disorders.
Fontana et al. (2018)
2 more

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