› 1j9s

X-ray diffraction
1.9Å resolution

Crystal structure of nitrite soaked oxidized H255N AFNIR

Released:
DOI: 10.2210/pdb1j9s/pdb
PDB entry 1j9s coloured by chain, front view.
PDB entry 1j9s coloured by chain, side view.
PDB entry 1j9s coloured by chain, top view.
Source organism: Alcaligenes faecalis
Primary publication:
Alternate substrate binding modes to two mutant (D98N and H255N) forms of nitrite reductase from Alcaligenes faecalis S-6: structural model of a transient catalytic intermediate.
Boulanger MJ, Murphy ME
Biochemistry 40 9132-41 (2001)
PMID: 11478880

Function and Biology Details

Reaction catalysed: 
Nitric oxide + H(2)O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153659 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Copper-containing nitrite reductase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 341 amino acids
Theoretical weight: 36.85 KDa
Source organism: Alcaligenes faecalis
Expression system: Escherichia coli
UniProt:
  • Canonical: P38501 (Residues: 40-376; Coverage: 98%)
Gene names: nir, nirK
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

2 bound ligands:
Ligand CU 6 x CU
Ligand NO2 3 x NO2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P212121
Unit cell:
a: 62.001Å b: 102.416Å c: 146.305Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.164 0.204
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Binding and activation of nitrite and nitric oxide by copper nitrite reductase and corresponding model complexes.
Merkle et al. (2012)
2 more

1 mention without citation

Copper active sites in biology.
Solomon et al. (2014)