› 1jok

Solution NMR

Averaged structure for Staphylococcal nuclease-H124L in ternary complex with Ca2+ and thymidine-3',5'-bisphosphate

Released:
DOI: 10.2210/pdb1jok/pdb
PDB entry 1jok coloured by chain, front view.
PDB entry 1jok coloured by chain, side view.
PDB entry 1jok coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Solution structures of staphylococcal nuclease from multidimensional, multinuclear NMR: nuclease-H124L and its ternary complex with Ca2+ and thymidine-3',5'-bisphosphate.
Wang J, Truckses DM, Abildgaard F, Dzakula Z, Zolnai Z, Markley JL
J Biomol NMR 10 143-64 (1997)
PMID: 9369015

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132790 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nuclease A Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 16.82 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00644 (Residues: 83-231; Coverage: 73%)
Gene name: nuc
Sequence domains: Staphylococcal nuclease homologue
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

1 bound ligand:
Ligand THP 1 x THP
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: Distance, hydrogen bond and torsion angle constraints
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Lessons from pressure denaturation of proteins.
Roche et al. (2018)
22 other articles

2 mentions without citation

Systematic comparison of crystal and NMR protein structures deposited in the protein data bank.
Sikic et al. (2010)
1 more