› 1k9t

X-ray diffraction
1.8Å resolution

Chitinase a complexed with tetra-N-acetylchitotriose

Released:
DOI: 10.2210/pdb1k9t/pdb
PDB entry 1k9t coloured by chain, front view.
PDB entry 1k9t coloured by chain, side view.
PDB entry 1k9t coloured by chain, top view.
Source organism: Serratia marcescens
Entry authors: Prag G, Tucker PA, Oppenheim AB

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139458 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Chitinase A Chain: A
Molecule details ›
Chain: A
Length: 540 amino acids
Theoretical weight: 58.6 KDa
Source organism: Serratia marcescens
Expression system: Escherichia coli
UniProt:
  • Canonical: P07254 (Residues: 24-563; Coverage: 100%)
Gene name: chiA
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: C2221
Unit cell:
a: 199.853Å b: 131.907Å c: 59.539Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.18 0.221
Expression system: Escherichia coli

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