› 1kmp

X-ray diffraction
2.5Å resolution

Crystal structure of the Outer Membrane Transporter FecA Complexed with Ferric Citrate

Released:
DOI: 10.2210/pdb1kmp/pdb
PDB entry 1kmp coloured by chain, front view.
PDB entry 1kmp coloured by chain, side view.
PDB entry 1kmp coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural basis of gating by the outer membrane transporter FecA.
Ferguson AD, Chakraborty R, Smith BS, Esser L, van der Helm D, Deisenhofer J
Science 295 1715-9 (2002)
PMID: 11872840

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146442 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fe(3+) dicitrate transport protein FecA Chain: A
Molecule details ›
Chain: A
Length: 774 amino acids
Theoretical weight: 86.11 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P13036 (Residues: 1-774; Coverage: 100%)
Gene names: JW4251, b4291, fecA
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand FE 2 x FE
Ligand CIT 2 x CIT
Ligand LDA 16 x LDA
1 modified residue:
Ligand MSE 15 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P21212
Unit cell:
a: 117.467Å b: 88.762Å c: 95.071Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.242 0.242 0.282
Expression system: Escherichia coli

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Citations

58 review citations

Bacterial iron homeostasis.
Andrews et al. (2003)
57 more

14 mentions without citation

Molecular basis of bacterial outer membrane permeability revisited.
Nikaido H. (2003)
13 more