› 1kpe

X-ray diffraction
1.8Å resolution

PKCI-TRANSITION STATE ANALOG

Released:
DOI: 10.2210/pdb1kpe/pdb
PDB entry 1kpe coloured by chain, front view.
PDB entry 1kpe coloured by chain, side view.
PDB entry 1kpe coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure-based analysis of catalysis and substrate definition in the HIT protein family.
Lima CD, Klein MG, Hendrickson WA
Science 278 286-90 (1997)
PMID: 9323207

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-546376 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenosine 5'-monophosphoramidase HINT1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 126 amino acids
Theoretical weight: 13.72 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P49773 (Residues: 2-126; Coverage: 99%)
Gene names: HINT, HINT1, PKCI1, PRKCNH1
Sequence domains: HIT domain
Structure domains: HIT-like

Ligands and Environments

1 bound ligand:
Ligand ADW 1 x ADW
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 46.2Å b: 77.8Å c: 80.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.195 0.223
Expression system: Komagataella pastoris

Quick links

Citations

30 review citations

Messenger RNA turnover in eukaryotes: pathways and enzymes.
Meyer et al. (2004)
29 more

5 mentions without citation

The catalytic scaffold of the haloalkanoic acid dehalogenase enzyme superfamily acts as a mold for the trigonal bipyramidal transition state.
Lu et al. (2008)
4 more