› 1lt5

X-ray diffraction
1.7Å resolution

HEAT-LABILE ENTEROTOXIN B-PENTAMER COMPLEXED WITH THIODIGALACTOSIDE

Released:
DOI: 10.2210/pdb1lt5/pdb
PDB entry 1lt5 coloured by chain, front view.
PDB entry 1lt5 coloured by chain, side view.
PDB entry 1lt5 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural foundation for the design of receptor antagonists targeting Escherichia coli heat-labile enterotoxin.
Merritt EA, Sarfaty S, Feil IK, Hol WG
Structure 5 1485-99 (1997)
PMID: 9384564

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152492 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Heat-labile enterotoxin B chain Chains: D, E, F, G, H
Molecule details ›
Chains: D, E, F, G, H
Length: 103 amino acids
Theoretical weight: 11.81 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P32890 (Residues: 22-124; Coverage: 100%)
Gene names: eltB, ltpB
Sequence domains: Heat-labile enterotoxin beta chain
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

Carbohydrate polymer : NEW Components: YIO, GAL
Ligand YIO 5 x YIO
Ligand GAL 5 x GAL
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P21
Unit cell:
a: 42.68Å b: 100.13Å c: 63.67Å
α: 90° β: 107.66° γ: 90°
R-values:
R R work R free
0.172 0.172 not available
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Lessons from lactose permease.
Guan et al. (2006)
8 more

2 mentions without citation

Peptide-plane flipping in proteins.
Hayward S. (2001)
1 more