› 1lu2

X-ray diffraction
2.8Å resolution

DOLICHOS BIFLORUS SEED LECTIN IN COMPLEX WITH THE BLOOD GROUP A TRISACCHARIDE

Released:
DOI: 10.2210/pdb1lu2/pdb
PDB entry 1lu2 coloured by chain, front view.
PDB entry 1lu2 coloured by chain, side view.
PDB entry 1lu2 coloured by chain, top view.
Source organism: Vigna cylindrica
Primary publication:
Carbohydrate binding, quaternary structure and a novel hydrophobic binding site in two legume lectin oligomers from Dolichos biflorus.
Hamelryck TW, Loris R, Bouckaert J, Dao-Thi MH, Strecker G, Imberty A, Fernandez E, Wyns L, Etzler ME
J Mol Biol 286 1161-77 (1999)
PMID: 10047489

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138337 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Seed lectin subunit I Chains: A, B
Molecule details ›
Chains: A, B
Length: 253 amino acids
Theoretical weight: 27.12 KDa
Source organism: Vigna cylindrica
Expression system: Escherichia coli
UniProt:
  • Canonical: P05045 (Residues: 23-275; Coverage: 100%)
Sequence domains: Legume lectin domain
Structure domains: Jelly Rolls

Ligands and Environments

3 bound ligands:
Ligand A2G 2 x A2G
Ligand CA 2 x CA
Ligand MN 2 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2
Unit cell:
a: 96.73Å b: 108Å c: 80.96Å
α: 90° β: 124.67° γ: 90°
R-values:
R R work R free
0.191 0.191 0.219
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Lectins.
Vijayan et al. (1999)
6 more

1 mention without citation

Glycoscience@Synchrotron: Synchrotron radiation applied to structural glycoscience.
Pérez et al. (2017)