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X-ray diffraction
2.2Å resolution

Crystal Structure of Cytochrome P450-cam with a Fluorescent Probe D-8-Ad (Adamantane-1-carboxylic acid-5-dimethylamino-naphthalene-1-sulfonylamino-octyl-amide)

Released:
DOI: 10.2210/pdb1lwl/pdb
PDB entry 1lwl coloured by chain, front view.
PDB entry 1lwl coloured by chain, side view.
PDB entry 1lwl coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
Fluorescent probes for cytochrome p450 structural characterization and inhibitor screening.
Dunn AR, Hays AM, Goodin DB, Stout CD, Chiu R, Winkler JR, Gray HB
J Am Chem Soc 124 10254-5 (2002)
PMID: 12197708

Function and Biology Details

Reaction catalysed: 
(+)-camphor + reduced putidaredoxin + O(2) = (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-523344 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Camphor 5-monooxygenase Chain: A
Molecule details ›
Chain: A
Length: 417 amino acids
Theoretical weight: 46.96 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: P00183 (Residues: 1-415; Coverage: 100%)
Gene names: camC, cyp101
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
1 bound ligand:
Ligand DSO 1 x DSO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 64.955Å b: 75.313Å c: 93.178Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.202 0.247
Expression system: Escherichia coli

Quick links

Citations

13 citation in other articles

Conformational states of cytochrome P450cam revealed by trapping of synthetic molecular wires.
Hays et al. (2004)
12 more

4 mentions without citation

Conformational plasticity and structure/function relationships in cytochromes P450.
Pochapsky et al. (2010)
3 more