› 1m7y

X-ray diffraction
1.6Å resolution

Crystal structure of apple ACC synthase in complex with L-aminoethoxyvinylglycine

Released:
DOI: 10.2210/pdb1m7y/pdb
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PDB entry 1m7y coloured by chain, front view.
PDB entry 1m7y coloured by chain, side view.
PDB entry 1m7y coloured by chain, top view.
Source organism: Malus domestica
Primary publication:
Apple 1-aminocyclopropane-1-carboxylate synthase in complex with the inhibitor L-aminoethoxyvinylglycine. Evidence for a ketimine intermediate.
Capitani G, McCarthy DL, Gut H, Grütter MG, Kirsch JF
J Biol Chem 277 49735-42 (2002)
PMID: 12228256

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine = 1-aminocyclopropane-1-carboxylate + S-methyl-5'-thioadenosine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153493 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1-aminocyclopropane-1-carboxylate synthase Chain: A
Molecule details ›
Chain: A
Length: 435 amino acids
Theoretical weight: 49.09 KDa
Source organism: Malus domestica
Expression system: Escherichia coli
UniProt:
  • Canonical: P37821 (Residues: 1-435; Coverage: 92%)
Gene name: ACS-1
Sequence domains: Aminotransferase class I and II
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand PPG 1 x PPG
Ligand MRD 1 x MRD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM1A
Spacegroup: C2
Unit cell:
a: 103.31Å b: 61.14Å c: 77.14Å
α: 90° β: 123.36° γ: 90°
R-values:
R R work R free
0.205 0.205 0.219
Expression system: Escherichia coli

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Citations

2 review citations

Pyridoxal phosphate enzymes: mechanistic, structural, and evolutionary considerations.
Eliot et al. (2004)
1 more

5 mentions without citation

Noncanonical Functions of Enzyme Cofactors as Building Blocks in Natural Product Biosynthesis.
Barra et al. (2022)
4 more

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