› 1mkv

X-ray diffraction
1.89Å resolution

CARBOXYLIC ESTER HYDROLASE COMPLEX (PLA2 + TRANSITION STATE ANALOG COMPLEX)

Released:
DOI: 10.2210/pdb1mkv/pdb
PDB entry 1mkv coloured by chain, front view.
PDB entry 1mkv coloured by chain, side view.
PDB entry 1mkv coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structure of the complex of bovine pancreatic phospholipase A2 with a transition-state analogue.
Sekar K, Kumar A, Liu X, Tsai MD, Gelb MH, Sundaralingam M
Acta Crystallogr D Biol Crystallogr 54 334-41 (1998)
PMID: 9761900

Function and Biology Details

Reaction catalysed: 
Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132751 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospholipase A2 Chain: A
Molecule details ›
Chain: A
Length: 123 amino acids
Theoretical weight: 13.81 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00593 (Residues: 23-145; Coverage: 95%)
Gene name: PLA2G1B
Sequence domains: Phospholipase A2
Structure domains: Phospholipase A2 domain

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand GEL 1 x GEL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SIEMENS
Spacegroup: P3121
Unit cell:
a: 46.58Å b: 46.58Å c: 102.91Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.18 0.18 not available
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Structure, function and interfacial allosterism in phospholipase A2: insight from the anion-assisted dimer.
Bahnson BJ. (2005)
9 other articles

5 mentions without citation

3dSS: 3D structural superposition.
Sumathi et al. (2006)
4 more