› 1mzy

X-ray diffraction
1.46Å resolution

Crystal Structure of Nitrite Reductase

Released:
DOI: 10.2210/pdb1mzy/pdb
Model geometry
Fit model/data
Data not deposited
PDB entry 1mzy coloured by chain, front view.
PDB entry 1mzy coloured by chain, side view.
PDB entry 1mzy coloured by chain, top view.
Source organism: Cereibacter sphaeroides
Entry authors: Guo H, Olesen K, Xue Y, Shapliegh J, Sjolin L

Function and Biology Details

Reaction catalysed: 
Nitric oxide + H(2)O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176049 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Copper-containing nitrite reductase Chain: A
Molecule details ›
Chain: A
Length: 333 amino acids
Theoretical weight: 36.34 KDa
Source organism: Cereibacter sphaeroides
Expression system: Escherichia coli
UniProt:
  • Canonical: Q53239 (Residues: 39-371; Coverage: 97%)
Gene names: Rsph17025_1595, nirK
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

2 bound ligands:
Ligand CU 2 x CU
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: R3
Unit cell:
a: 71.392Å b: 71.392Å c: 146.246Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.185 0.21
Expression system: Escherichia coli

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