› 1n2l

X-ray diffraction
3.2Å resolution

Crystal structure of a covalent intermediate of endogenous human arylsulfatase A

Released:
DOI: 10.2210/pdb1n2l/pdb
PDB entry 1n2l coloured by chain, front view.
PDB entry 1n2l coloured by chain, side view.
PDB entry 1n2l coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of a covalent intermediate of endogenous human arylsulfatase A.
Chruszcz M, Laidler P, Monkiewicz M, Ortlund E, Lebioda L, Lewinski K
J Inorg Biochem 96 386-92 (2003)
PMID: 12888274

Function and Biology Details

Reaction catalysed: 
A cerebroside 3-sulfate + H(2)O = a cerebroside + sulfate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147294 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Arylsulfatase A Chain: A
Molecule details ›
Chain: A
Length: 489 amino acids
Theoretical weight: 52.04 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P15289 (Residues: 19-507; Coverage: 100%)
Gene name: ARSA
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand NAG 1 x NAG
Ligand CA 1 x CA
1 modified residue:
Ligand FGP 1 x FGP

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: I422
Unit cell:
a: 131.25Å b: 131.25Å c: 191.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.182 0.223

Quick links

Citations

5 review citations

Sulfatases: structure, mechanism, biological activity, inhibition, and synthetic utility.
Hanson et al. (2004)
4 more

2 mentions without citation

Structure of sulfamidase provides insight into the molecular pathology of mucopolysaccharidosis IIIA.
Sidhu et al. (2014)
1 more