› 1nbm

X-ray diffraction
3Å resolution

THE STRUCTURE OF BOVINE F1-ATPASE COVALENTLY INHIBITED WITH 4-CHLORO-7-NITROBENZOFURAZAN

Released:
DOI: 10.2210/pdb1nbm/pdb
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PDB entry 1nbm coloured by chain, front view.
PDB entry 1nbm coloured by chain, side view.
PDB entry 1nbm coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Bovine F1-ATPase covalently inhibited with 4-chloro-7-nitrobenzofurazan: the structure provides further support for a rotary catalytic mechanism.
Orriss GL, Leslie AG, Braig K, Walker JE
Structure 6 831-7 (1998)
PMID: 9687365

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + 4 H(+)(Side 1) = ADP + phosphate + 4 H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133609 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
ATP synthase F(1) complex subunit alpha, mitochondrial Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 510 amino acids
Theoretical weight: 55.3 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P19483 (Residues: 44-553; Coverage: 92%)
Gene names: ATP5A1, ATP5A2, ATP5F1A
Sequence domains:
Structure domains:
ATP synthase F(1) complex catalytic subunit beta, mitochondrial Chains: D, F
Molecule details ›
Chains: D, F
Length: 480 amino acids
Theoretical weight: 51.53 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00829 (Residues: 47-526; Coverage: 91%)
Gene names: ATP5B, ATP5F1B
Sequence domains:
Structure domains:
ATP synthase F(1) complex catalytic subunit beta, mitochondrial Chain: E
ATP synthase F(1) complex subunit gamma, mitochondrial Chain: G

Ligands and Environments

4 bound ligands:
Ligand MG 5 x MG
Ligand ATP 4 x ATP
Ligand ADP 1 x ADP
Ligand PO4 1 x PO4
1 modified residue:
Ligand TYN 1 x TYN

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P212121
Unit cell:
a: 281Å b: 106.6Å c: 138.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.207 0.297

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Citations

16 review citations

The ATP synthase: the understood, the uncertain and the unknown.
Walker JE. (2013)
15 more

4 mentions without citation

Automated ligand fitting by core-fragment fitting and extension into density.
Terwilliger et al. (2006)
3 more

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