› 1o6k

X-ray diffraction
1.7Å resolution

Structure of activated form of PKB kinase domain S474D with GSK3 peptide and AMP-PNP

Released:
DOI: 10.2210/pdb1o6k/pdb
PDB entry 1o6k coloured by chain, front view.
PDB entry 1o6k coloured by chain, side view.
PDB entry 1o6k coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of an activated Akt/protein kinase B ternary complex with GSK3-peptide and AMP-PNP.
Yang J, Cron P, Good VM, Thompson V, Hemmings BA, Barford D
Nat Struct Biol 9 940-4 (2002)
PMID: 12434148

Function and Biology Details

Reactions catalysed: 
ATP + a protein = ADP + a phosphoprotein
ATP + [tau protein] = ADP + [tau protein] phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152088 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
RAC-beta serine/threonine-protein kinase Chain: A
Molecule details ›
Chain: A
Length: 336 amino acids
Theoretical weight: 39.12 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P31751 (Residues: 146-481; Coverage: 70%)
Gene name: AKT2
Sequence domains:
Structure domains:
Glycogen synthase kinase-3 beta Chain: C
Molecule details ›
Chain: C
Length: 10 amino acids
Theoretical weight: 1.12 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P49841 (Residues: 3-12; Coverage: 2%)
Gene name: GSK3B

Ligands and Environments

2 bound ligands:
Ligand ANP 1 x ANP
Ligand MN 2 x MN
1 modified residue:
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 44.906Å b: 60.998Å c: 129.41Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.205 0.234
Expression system: Spodoptera frugiperda

Quick links

Citations

66 review citations

AKT/PKB signaling: navigating downstream.
Manning et al. (2007)
65 more

56 mentions without citation

Catalytic control in the EGF receptor and its connection to general kinase regulatory mechanisms.
Jura et al. (2011)
55 more