› 1pg5

X-ray diffraction
2.6Å resolution

CRYSTAL STRUCTURE OF THE UNLIGATED (T-STATE) ASPARTATE TRANSCARBAMOYLASE FROM THE EXTREMELY THERMOPHILIC ARCHAEON SULFOLOBUS ACIDOCALDARIUS

Released:
DOI: 10.2210/pdb1pg5/pdb
Model geometry
Fit model/data
PDB entry 1pg5 coloured by chain, front view.
PDB entry 1pg5 coloured by chain, side view.
PDB entry 1pg5 coloured by chain, top view.
Source organism: Sulfolobus acidocaldarius
Primary publication:
Crystal structure of T state aspartate carbamoyltransferase of the hyperthermophilic archaeon Sulfolobus acidocaldarius.
De Vos D, Van Petegem F, Remaut H, Legrain C, Glansdorff N, Van Beeumen JJ
J Mol Biol 339 887-900 (2004)
PMID: 15165857

Function and Biology Details

Reaction catalysed: 
Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159949 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Aspartate carbamoyltransferase catalytic subunit Chain: A
Molecule details ›
Chain: A
Length: 299 amino acids
Theoretical weight: 34.2 KDa
Source organism: Sulfolobus acidocaldarius
Expression system: Escherichia coli
UniProt:
  • Canonical: Q55338 (Residues: 1-299; Coverage: 100%)
Gene names: Saci_1596, pyrB
Sequence domains:
Structure domains: Aspartate/ornithine carbamoyltransferase
Aspartate carbamoyltransferase regulatory chain Chain: B
Molecule details ›
Chain: B
Length: 168 amino acids
Theoretical weight: 18.85 KDa
Source organism: Sulfolobus acidocaldarius
Expression system: Escherichia coli
UniProt:
  • Canonical: P74766 (Residues: 1-164; Coverage: 100%)
Gene names: Saci_1595, pyrI
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P6322
Unit cell:
a: 132.853Å b: 132.853Å c: 140.279Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.193 0.239
Expression system: Escherichia coli

Quick links

Citations

1 review citation

From Genome to Structure and Back Again: A Family Portrait of the Transcarbamylases.
Shi et al. (2015)
5 other articles

2 mentions without citation

Dihydroorotase from the hyperthermophile Aquifex aeolicus is activated by stoichiometric association with aspartate transcarbamoylase and forms a one-pot reactor for pyrimidine biosynthesis.
Zhang et al. (2009)
1 more