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Solution NMR

Solution Structure of the MutT Pyrophosphohydrolase Complexed with Mg(2+) and 8-oxo-dGMP, a Tightly-bound Product

Released:
DOI: 10.2210/pdb1ppx/pdb
PDB entry 1ppx coloured by chain, ensemble of 20 models, front view.
PDB entry 1ppx coloured by chain, ensemble of 20 models, side view.
PDB entry 1ppx coloured by chain, ensemble of 20 models, top view.
Source organism: Escherichia coli
Primary publication:
Solution structure and NH exchange studies of the MutT pyrophosphohydrolase complexed with Mg(2+) and 8-oxo-dGMP, a tightly bound product.
Massiah MA, Saraswat V, Azurmendi HF, Mildvan AS
Biochemistry 42 10140-54 (2003)
PMID: 12939141

Function and Biology Details

Reaction catalysed: 
8-oxo-dGTP + H(2)O = 8-oxo-dGMP + diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140099 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
8-oxo-dGTP diphosphatase Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.95 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P08337 (Residues: 1-129; Coverage: 100%)
Gene names: JW0097, b0099, mutT
Sequence domains: NUDIX domain
Structure domains: Nucleoside Triphosphate Pyrophosphohydrolase

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand 8OG 1 x 8OG
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing torsion angle dynamics
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Structures and mechanisms of Nudix hydrolases.
Mildvan et al. (2005)
3 more

4 mentions without citation

Structural basis of UGUA recognition by the Nudix protein CFI(m)25 and implications for a regulatory role in mRNA 3' processing.
Yang et al. (2010)
3 more