› 1qb0

X-ray diffraction
1.91Å resolution

HUMAN CDC25B CATALYTIC DOMAIN

Released:
DOI: 10.2210/pdb1qb0/pdb
PDB entry 1qb0 coloured by chain, front view.
PDB entry 1qb0 coloured by chain, side view.
PDB entry 1qb0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the catalytic subunit of Cdc25B required for G2/M phase transition of the cell cycle.
Reynolds RA, Yem AW, Wolfe CL, Deibel MR, Chidester CG, Watenpaugh KD
J Mol Biol 293 559-68 (1999)
PMID: 10543950

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151701 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
M-phase inducer phosphatase 2 Chain: A
Molecule details ›
Chain: A
Length: 211 amino acids
Theoretical weight: 24.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P30305 (Residues: 370-580; Coverage: 36%)
Gene names: CDC25B, CDC25HU2
Sequence domains: Rhodanese-like domain
Structure domains: Rhodanese-like domain

Ligands and Environments

3 bound ligands:
Ligand SO4 2 x SO4
Ligand CL 1 x CL
Ligand BME 2 x BME
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SIEMENS
Spacegroup: P212121
Unit cell:
a: 51.169Å b: 71.097Å c: 73.515Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.143 not available not available
Expression system: Escherichia coli

Quick links

Citations

26 review citations

Cdc25 phosphatases and cancer.
Kristjánsdóttir et al. (2004)
25 more

26 mentions without citation

Sulfenic acid chemistry, detection and cellular lifetime.
Gupta et al. (2014)
25 more