› 1qy4

X-ray diffraction
1.8Å resolution

Crystal structure of phosphoglucose isomerase from Pyrococcus furiosus in complex with gluconate 6-phosphate

Released:
DOI: 10.2210/pdb1qy4/pdb
PDB entry 1qy4 coloured by chain, front view.
PDB entry 1qy4 coloured by chain, side view.
PDB entry 1qy4 coloured by chain, top view.
Source organism: Pyrococcus furiosus
Primary publication:
Structural evidence for a hydride transfer mechanism of catalysis in phosphoglucose isomerase from Pyrococcus furiosus.
Swan MK, Solomons JT, Beeson CC, Hansen T, Schönheit P, Davies C
J Biol Chem 278 47261-8 (2003)
PMID: 12970347

Function and Biology Details

Reaction catalysed: 
Alpha-D-glucose 6-phosphate = beta-D-fructofuranose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160676 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose-6-phosphate isomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 189 amino acids
Theoretical weight: 21.51 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P83194 (Residues: 1-189; Coverage: 100%)
Gene names: PF0196, pgiA
Sequence domains: Glucose-6-phosphate isomerase (GPI)
Structure domains: Jelly Rolls

Ligands and Environments

2 bound ligands:
Ligand 6PG 2 x 6PG
Ligand NI 2 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P1
Unit cell:
a: 36Å b: 39.7Å c: 74.7Å
α: 73.7° β: 78.5° γ: 72.6°
R-values:
R R work R free
0.22 0.218 0.266
Expression system: Escherichia coli BL21

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Citations

3 review citations

Structural studies on 2-oxoglutarate oxygenases and related double-stranded beta-helix fold proteins.
Clifton et al. (2006)
2 more

2 mentions without citation

Divergence and convergence in enzyme evolution.
Galperin et al. (2012)
1 more