› 1s4r

X-ray diffraction
1.9Å resolution

Structure of a reaction intermediate in the photocycle of PYP extracted by a SVD-driven analysis

Released:
DOI: 10.2210/pdb1s4r/pdb
PDB entry 1s4r coloured by chain, front view.
PDB entry 1s4r coloured by chain, side view.
PDB entry 1s4r coloured by chain, top view.
Source organism: Halorhodospira halophila
Primary publication:
Protein kinetics: structures of intermediates and reaction mechanism from time-resolved x-ray data.
Schmidt M, Pahl R, Srajer V, Anderson S, Ren Z, Ihee H, Rajagopal S, Moffat K
Proc Natl Acad Sci U S A 101 4799-804 (2004)
PMID: 15041745

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Photoactive yellow protein Chain: A
Molecule details ›
Chain: A
Length: 125 amino acids
Theoretical weight: 13.89 KDa
Source organism: Halorhodospira halophila
Expression system: Escherichia coli
UniProt:
  • Canonical: P16113 (Residues: 1-125; Coverage: 100%)
Gene name: pyp
Sequence domains: PAS fold
Structure domains: PAS domain

Ligands and Environments

1 bound ligand:
Ligand HC4 1 x HC4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B
Spacegroup: P63
Unit cell:
a: 66.9Å b: 66.9Å c: 40.8Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.242 0.242 0.259
Expression system: Escherichia coli

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Citations

7 review citations

Integrative, dynamic structural biology at atomic resolution--it's about time.
van den Bedem et al. (2015)
6 more

1 mention without citation

Protein kinetics: structures of intermediates and reaction mechanism from time-resolved x-ray data.
Schmidt et al. (2004)