1s5n

X-ray diffraction
0.95Å resolution

Xylose Isomerase in Substrate and Inhibitor Michaelis States: Atomic Resolution Studies of a Metal-Mediated Hydride Shift

Released:

Function and Biology Details

Reaction catalysed:
Alpha-D-xylopyranose = alpha-D-xylufuranose
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147411 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Xylose isomerase Chain: A
Molecule details ›
Chain: A
Length: 386 amino acids
Theoretical weight: 42.86 KDa
Source organism: Streptomyces olivochromogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: P15587 (Residues: 2-387; Coverage: 100%)
Gene name: xylA
Sequence domains: Xylose isomerase-like TIM barrel
Structure domains: Divalent-metal-dependent TIM barrel enzymes

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: I222
Unit cell:
a: 86.031Å b: 92.854Å c: 98.312Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.106 0.106 0.128
Expression system: Escherichia coli