› 1sn8

X-ray diffraction
2Å resolution

Crystal structure of the S1 domain of RNase E from E. coli (Pb derivative)

Released:
DOI: 10.2210/pdb1sn8/pdb
PDB entry 1sn8 coloured by chain, front view.
PDB entry 1sn8 coloured by chain, side view.
PDB entry 1sn8 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural characterization of the RNase E S1 domain and identification of its oligonucleotide-binding and dimerization interfaces.
Schubert M, Edge RE, Lario P, Cook MA, Strynadka NC, Mackie GA, McIntosh LP
J Mol Biol 341 37-54 (2004)
PMID: 15312761

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149271 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease E Chains: A, B
Molecule details ›
Chains: A, B
Length: 96 amino acids
Theoretical weight: 10.7 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P21513 (Residues: 35-125; Coverage: 9%)
Gene names: JW1071, ams, b1084, hmp1, rne
Sequence domains: S1 RNA binding domain
Structure domains: Nucleic acid-binding proteins

Ligands and Environments

1 bound ligand:
Ligand PB 2 x PB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P41212
Unit cell:
a: 70.58Å b: 70.58Å c: 87.902Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.182 0.232
Expression system: Escherichia coli BL21

Quick links

Citations

6 review citations

RNA-binding proteins: modular design for efficient function.
Lunde et al. (2007)
5 more