Function and Biology Details
Reaction catalysed:
Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides.
Biochemical function:
Biological process:
Cellular component:
- not assigned
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
Subtilisin BPN' and peptide (preferred)
PDBe Complex ID:
PDB-CPX-133534 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Subtilisin BPN'
Molecule details ›
Chain: A
Length: 266 amino acids
Theoretical weight: 26.62 KDa
Source organism: Bacillus amyloliquefaciens
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Subtilase family
Structure domains: Peptidase S8/S53 domain
Length: 266 amino acids
Theoretical weight: 26.62 KDa
Source organism: Bacillus amyloliquefaciens
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P00782 (Residues: 108-382; Coverage: 76%)
Sequence domains: Subtilase family
Structure domains: Peptidase S8/S53 domain
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
RIGAKU RUH2R
Spacegroup:
P212121
Expression systems:
- Escherichia coli BL21(DE3)
- Not provided
